In vivo label-free quantification of liver microcirculation using dual-modality microscopy
Yan,Jie Kang,Yuzhan Xu,Shuoyu Ong,Lee Ling S. Zhuo,Shuangmu Bunte,Ralph M. Chen,Nanguang Asada,H. Harry So,Peter T.C. Wanless,Ian R.
Yan,Jie
Kang,Yuzhan
Xu,Shuoyu
Ong,Lee Ling S.
Zhuo,Shuangmu
Bunte,Ralph M.
Chen,Nanguang
Asada,H. Harry
So,Peter T.C.
Wanless,Ian R.
Abstract
Microcirculation lesion is a common symptom of chronic liver diseases in the form of vasculature deformation and circulation alteration. In acute to chronic liver diseases such as biliary atresia, microcirculation lesion can have an early onset. Detection of microcirculation lesion is meaningful for studying the progression of liver disease. We have combined wide-field fluorescence microscopy and a laser speckle contrast technique to characterize hepatic microcirculation in vivo without labeling in a bile-duct ligation rat fibrosis model of biliary atresia. Through quantitative image analysis of four microcirculation parameters, we observed significant microcirculation lesion in the early to middle stages of fibrosis. This bimodal imaging method is useful to assess hepatic microcirculation lesion for the study of liver diseases.
Description
Publisher Copyright: © 2014 Society of Photo-Optical Instrumentation Engineers (SPIE).
Date
2014-11-01
Journal Title
Journal ISSN
Volume Title
Publisher
Research Projects
Organizational Units
Journal Issue
Keywords
Hepatic microcirculation, Laser speckle contrast imaging, Wide-field fluorescence microscopy
Citation
Yan, J, Kang, Y, Xu, S, Ong, L L S, Zhuo, S, Bunte, R M, Chen, N, Asada, H H, So, P T C, Wanless, I R & Yu, H 2014, 'In vivo label-free quantification of liver microcirculation using dual-modality microscopy', Journal of Biomedical Optics, vol. 19, no. 11, 116006. https://doi.org/10.1117/1.JBO.19.11.116006